ISO INTERNATIONAL STANDARD 19929 First edition 2017-03 Plastics Determination of average molecular mass and mixture ratio of poly(ethylene glycol) and its derivatives by MALDI-TOF-MS Plastiques - Détermination de la masse moleculaire moyenne et du rapport de mélange du poly(éthylene glycol) et de ses dérivés par MALDI-TOF-MS Reference number IS0 19929:2017(E) International Organization for Standardization @ IS0 2017 ZHEJIANG INSTOF STANDARDIZATION C15956617 ted without license from IHS IS0 19929:2017(E) COPYRIGHTPROTECTEDDOCUMENT IS0 2017, Published in Switzerland All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below or ISO's member body in the country of the requester. ISOcopyrightoffice Ch. de Blandonnet 8 · CP 401 CH-1214 Vernier, Geneva, Switzerland Tel. +41 22 749 01 11 Fax +41 22 749 09 47 [email protected] www.iso.org Internationai ibrg DrganizationforStandardization Licensee-ZHEJIANG INST.OF STANDARDIZzRoISQ7 - All rights reserved Not for Resale, 2017/5/16 00:47:50 networking permited withoutlicense from IHS IS0 19929:2017(E) Contents Page Foreword ..iv Introduction. ..V 1 Scope. ..1 2 Normative references 3 Terms and definitions 4 Principles 5 Reagents. 6 Apparatus. 7 Procedure ..5 7.1 General ..5 7.2 Sample preparation .5 7.2.1 General ..5 7.2.2 Preparation of polymer standard mixtures .6 7.2.3 Preparation of polymer/matrix/salt solutions .6 7.2.4 Deposition of the sample on the sample plate (target) ..6 7.2.5 Preparation of biopolymer/matrix solutions 7 7.3 Instrument settings 7.4 Spectra recording.. ..8 Data acquisition and processing .8 8 8.1 General .8 8.2 Calibration of mass axis. ..8 8.2.1 General 8 8.2.2 Calibration of mass axis using synthetic polymer standards .9 8.2.3 Calibration of mass axis using biopolymer standards 9 8.2.4 Self-calibration method .9 8.3 Generation of mass calibration curve. .9 8.4 Calibration of intensity axis. .9 9 Expression of results .10 9.1 Calculation of molecular mass distribution (MMD) .10 9.2 Calculation of the number-average molecular mass .10 9.3 Calculation of peak area... ..10 9.4 Constructing calibration curve for intensity 10 9.5 Calculation of mass ratio. .11 10 Precision ..11 11 Test report. ..11 11.1 General .11 11.2 Apparatus .11 11.3 Calibration 11 11.4 Results ..11 Annex A (normative) Calibrants. ..12 Bibliography ..13 ii icensee=ZHEJIANG INST OF STANDARDIZATION C1 5956617 ed without license from IHS